Haas Kurt, Jensen Kendall, Sin Wun Chey, Foa Lisa, Cline Hollis T
Differentiation. 2002 Jun;70(4-5):148-54. doi: 10.1046/j.1432-0436.2002.700404.x.
Electroporation is becoming more popular as a technique for transfecting neurons within intact tissues. One of the advantages of electroporation over other transfection techniques is the ability to precisely target an area for transfection. Here we highlight this advantage by describing methods to restrict transfection to either a single cell, clusters of cells, or to include large portions of the brain of the intact Xenopus tadpole. Electroporation is also an effective means of gene delivery in the retina. We have developed these techniques to examine the effects of regulated gene expression on various neuronal properties, including structural plasticity and synaptic transmission. Restriction of transfection to individual cells aids in imaging of neuronal morphology, while bulk cell transfection allows examination of the affects of gene expression on populations of cells by biochemical assays, imaging, and electrophysiological recording.
电穿孔作为一种在完整组织中转染神经元的技术正变得越来越流行。与其他转染技术相比，电穿孔的优势之一是能够精确地靶向转染区域。在这里，我们通过描述将转染限制在单个细胞、细胞簇或包括完整非洲爪蟾蝌蚪大脑的大部分区域的方法来突出这一优势。电穿孔也是视网膜中基因递送的有效手段。我们开发了这些技术来研究基因表达调控对各种神经元特性的影响，包括结构可塑性和突触传递。将转染限制在单个细胞有助于神经元形态的成像，而大量细胞转染则允许通过生化分析、成像和电生理记录来研究基因表达对细胞群体的影响。
