Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Center, La Jolla, California 92039, USA
Cold Spring Harb Protoc. 2022 Feb 1;2022(2):pdb.top106773. doi: 10.1101/pdb.top106773.
In vivo time-lapse imaging has been a fruitful approach to identify structural and functional changes in the nervous system in tadpoles and adult frogs. Structural imaging studies have identified fundamental aspects of brain connectivity, development, plasticity, and disease and have been instrumental in elucidating mechanisms regulating these events in vivo. Similarly, assessment of nervous system function using dynamic changes in calcium signals as a proxy for neuronal activity has demonstrated principles of neuron and circuit function and principles of information organization and transfer within the brain of living animals. Because of its many advantages as an experimental system, use of has often been at the forefront of developing these imaging methods for in vivo applications. Protocols for in vivo structural and functional imaging-including cellular labeling strategies, image collection, and image analysis-will expand the use of to understand brain development, function, and plasticity.
在体延时成像一直是一种有效的方法，可以识别蝌蚪和成年青蛙神经系统中的结构和功能变化。结构成像研究已经确定了大脑连接、发育、可塑性和疾病的基本方面，并有助于阐明体内调节这些事件的机制。同样，使用钙信号的动态变化作为神经元活动的替代物来评估神经系统功能，已经证明了神经元和电路功能的原理，以及大脑内信息组织和传递的原理。由于其作为实验系统的诸多优势， 使用通常处于开发这些用于体内应用的成像方法的前沿。包括细胞标记策略、图像采集和图像分析在内的体内结构和功能成像的方案将扩大 的使用范围，以了解大脑的发育、功能和可塑性。
