[多线染色体的DNP纤维中的DNA包装]
[DNA packing in the DNP fibrils of polytene chromosomes].
作者信息
Gruzdev A D, Kishchenko G P
出版信息
Biofizika. 1978 Nov-Dec;23(6):1000-4.
PMID:719012
Abstract
Isolated polytene chromosomes stained with acridine orange were stretched with micromanipulator needles. Changes of chromosome fluorescence polarization led to the conclusions: 1) almost complete orientation of DNP fibers is attained at very low levels of chromosome stretching (3--8 times) without irreversible disruption of chromomeres; 2) internucleosomal DNA in native DNP fibers is supercoiled and makes 30 degrees angle to the fiber axis.
摘要
用吖啶橙染色的孤立多线染色体用显微操作针拉伸。染色体荧光偏振的变化得出以下结论：1）在极低的染色体拉伸水平（3 - 8倍）下，DNP纤维几乎完全定向，且染色粒不会发生不可逆的破坏；2）天然DNP纤维中的核小体间DNA是超螺旋的，与纤维轴成30度角。
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