沙眼衣原体诊断检测核酸扩增试验的外部质量评估计划
External quality assessment program for Chlamydia trachomatis diagnostic testing by nucleic acid amplification assays.
作者信息
Land Sally, Tabrizi Sepehr, Gust Anthony, Johnson Elizabeth, Garland Susan, Dax Elizabeth M
机构信息
National Serology Reference Laboratory, Australia, St Vincent's Institute of Medical Research, Fitzroy, VIC 3065, Australia.
出版信息
J Clin Microbiol. 2002 Aug;40(8):2893-6. doi: 10.1128/JCM.40.8.2893-2896.2002.
Abstract
We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis-positive clinical samples. Only at the lower limit of detection of the assays did the R-PCR demonstrate increased sensitivity over the A-LCR in the detection of C. trachomatis antigen. However, it was found that single-sample testing could lead to decreased test sensitivity. Detection of the presence of inhibitors of nucleic acid amplification differed between laboratories.
摘要
我们报告了来自57家澳大利亚诊断实验室的结果，这些实验室使用罗氏Amplicor沙眼衣原体检测法（R-PCR）或雅培LCx沙眼衣原体检测法（A-连接酶链反应[LCR]）对两个外部质量评估样本组进行检测。样本组样本要么是添加了沙眼衣原体抗原的正常尿液，要么是临床尿液标本。在检测沙眼衣原体阳性临床样本方面，各实验室之间或各检测方法之间没有显著差异。仅在各检测方法的检测下限处，R-PCR在检测沙眼衣原体抗原方面显示出比A-LCR更高的灵敏度。然而，发现单样本检测可能导致检测灵敏度降低。各实验室在核酸扩增抑制剂存在的检测方面存在差异。
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2
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Curr Clin Top Infect Dis. 1998;18:37-59.
3
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