Baucheron Sylvie, Grayon Maggy, Zygmunt Michel S, Cloeckaert Axel
Institut National de la Recherche Agronomique, Unité de Pathologie Aviaire et Parasitologie, Nouzilly, France.
Res Microbiol. 2002 Jun;153(5):277-80. doi: 10.1016/s0923-2508(02)01317-7.
Smooth lipopolysaccharides (S-LPSs) from Brucella strains isolated from seals, dolphins, porpoises, an otter and a minke whale were characterized by ELISA using monoclonal antibodies (mAbs) directed against seven previously defined O-polysaccharide (O-PS) epitopes and by Western blot after SDS-PAGE. All strains studied were A-dominant as shown by specific polyclonal sera and this was also confirmed by the mAbs. However, binding patterns in ELISA of mAbs to the specific common (C) epitopes were rather heterogeneous, and for some strains, such as those isolated from striped dolphins, binding of these mAbs was much reduced or negative as had previously been shown for Brucella suis biovar 2 strains. Western blot after SDS-PAGE showed the typical A-dominant strain banding pattern for all marine mammal Brucella isolates, but the average S-LPS size was shorter in many of these compared to reference Brucella abortus strain 544. Thus, S-LPSs of the marine mammal isolates show heterogeneity with regard to their O-PS C epitope content and their average size.
利用针对7种先前定义的O-多糖（O-PS）表位的单克隆抗体（mAb），通过酶联免疫吸附测定（ELISA），并在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后进行蛋白质印迹法，对从海豹、海豚、鼠海豚、一只水獭和一头小须鲸分离出的布鲁氏菌菌株的光滑脂多糖（S-LPS）进行了特性分析。如特异性多克隆血清所示，所有研究的菌株均以A为主，单克隆抗体也证实了这一点。然而，单克隆抗体与特异性共同（C）表位在ELISA中的结合模式差异较大，对于一些菌株，如从条纹海豚分离出的菌株，这些单克隆抗体的结合大大减少或呈阴性，正如先前对猪布鲁氏菌生物变种2菌株所显示的那样。SDS-PAGE后的蛋白质印迹法显示，所有海洋哺乳动物布鲁氏菌分离株均呈现典型的A为主的菌株条带模式，但与参考布鲁氏菌流产菌株544相比，其中许多菌株的S-LPS平均大小较短。因此，海洋哺乳动物分离株的S-LPS在其O-PS C表位含量及其平均大小方面表现出异质性。
