Kittaka-Katsura Hiromi, Fujita Tomoyuki, Watanabe Fumio, Nakano Yoshihisa
Department of Health Science, Hiroshima Prefectural Women's University, Hiroshima 734-8558, Japan.
J Agric Food Chem. 2002 Aug 14;50(17):4994-7. doi: 10.1021/jf020345w.
Vitamin B(12) content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 microg/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 microg/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy.
采用赖氏乳酸杆菌ATCC 7830微生物法和内因子-化学发光法测定了一种藻类健康食品小球藻片（小球藻属）中的维生素B12含量。化学发光法测定的结果为200.9 - 211.6微克/100克干重，与微生物法测定的值（201.3 - 285.7微克/100克干重）相似。从小球藻片中纯化出一种类咕啉化合物并进行了表征。基于硅胶60薄层层析、C18反相高效液相色谱、1H核磁共振光谱和紫外-可见光谱，将纯化的类咕啉化合物鉴定为维生素B12。
