Watanabe Fumio, Tanioka Yuri, Miyamoto Emi, Fujita Tomoyuki, Takenaka Hiroyuki, Nakano Yoshihisa
School of Agricultural, Biological, and Environmental Sciences, Tottori University, Tottori 680-8553, Japan.
J Nutr Sci Vitaminol (Tokyo). 2007 Apr;53(2):183-6. doi: 10.3177/jnsv.53.183.
Vitamin B12 content (98.8 +/- 5.6 microg/100 g dry weight) of an edible cyanobacterium, Nostoc commune (Ishikurage) was determined by the Lactobacillus delbrueckii ATCC 7830 microbiological method. Bioautography with vitamin B12-dependent Escherichia coli 215 indicated that N. commune contained two (main and minor) corrinoid-compounds. These corrinoid-compounds were purified to homogeneity from the dried algal cells and characterized. The main and minor purified corrinoid-compounds were identified as pseudovitamin B12 and vitamin B12, respectively, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-visible spectroscopy. These results suggest that the bacterial cells are not suitable for use as a vitamin B12 source, especially in vegetarians.
采用德氏乳杆菌ATCC 7830微生物学方法测定了可食用蓝藻普通念珠藻（地木耳）的维生素B12含量（98.8±5.6微克/100克干重）。用依赖维生素B12的大肠杆菌215进行生物自显影表明，普通念珠藻含有两种（主要和次要）类咕啉化合物。这些类咕啉化合物从干燥的藻细胞中纯化至同质并进行了表征。基于硅胶60薄层色谱、C18反相高效液相色谱、1H核磁共振光谱和紫外可见光谱，主要和次要的纯化类咕啉化合物分别被鉴定为假维生素B12和维生素B12。这些结果表明，细菌细胞不适合用作维生素B12的来源，尤其是对素食者而言。
