Miyamoto Emi, Tanioka Yuri, Nakao Tomoyuki, Barla Florin, Inui Hiroshi, Fujita Tomoyuki, Watanabe Fumio, Nakano Yoshihisa
Department of Health and Nutrition, Nagasaki International University, Sasebo, Japan.
J Agric Food Chem. 2006 Dec 13;54(25):9604-7. doi: 10.1021/jf062300r.
The vitamin B12 concentration of the dried cells of Aphanizomenon flos-aquae was determined by both microbiological method with Lactobacillus delbrueckeii ATCC7830 and chemiluminescence method with intrinsic factor. The Aphanizomenon cells contained 616.3 +/- 30.3 micro g (n = 4) of vitamin B12 per 100 g of the dried cells by the microbiological method. The values determined with the chemiluminescence method, however, were only about 5.3% of the values determined by the microbiological method. A corrinoid-compound was purified from the dried cells and characterized. The purified corrinoid-compound was identified as pseudovitamin B12 (an inactive corrinoid-compound for humans) by silica gel 60 TLC, C18 reversed-phase HPLC, ultraviolet-visible spectroscopy, and 1H NMR spectroscopy. The results suggest that the Aphanizomenon cells are not suitable for use as a vitamin B12 source, especially in vegans.
采用德氏乳杆菌ATCC7830微生物法和内因子化学发光法测定了水华束丝藻干燥细胞中的维生素B12浓度。通过微生物法测定，每100克水华束丝藻干燥细胞中含有616.3±30.3微克（n = 4）的维生素B12。然而，化学发光法测定的值仅约为微生物法测定值的5.3%。从干燥细胞中纯化并表征了一种类咕啉化合物。通过硅胶60 TLC、C18反相HPLC、紫外可见光谱和1H NMR光谱，将纯化的类咕啉化合物鉴定为假维生素B12（一种对人类无活性的类咕啉化合物）。结果表明，水华束丝藻细胞不适宜用作维生素B12的来源，尤其是对于纯素食者。
