Sung K B, Liang C, Descour M, Collier T, Follen M, Malpica A, Richards-Kortum R
Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712, USA.
J Microsc. 2002 Aug;207(Pt 2):137-45. doi: 10.1046/j.1365-2818.2002.01049.x.
We have built a fibre optic confocal reflectance microscope capable of imaging biological tissue in near real time. The measured lateral resolution is 3 micro m and axial resolution is 6 micro m. Images of epithelial cells, excised tissue biopsies, and the human lip in vivo have been obtained at 15 frames s-1. Both cell morphology and tissue architecture can be appreciated from images obtained with this microscope. This device has the potential to enable reflected light confocal imaging of internal organs for in situ detection of pathology.
我们构建了一台能够对生物组织进行近实时成像的光纤共焦反射显微镜。所测得的横向分辨率为3微米，轴向分辨率为6微米。已在15帧/秒的速度下获取了上皮细胞、切除组织活检样本以及人体唇部的体内图像。通过该显微镜获得的图像能够清晰呈现细胞形态和组织结构。这一设备具有实现对内部器官进行反射光共焦成像以原位检测病理学特征的潜力。
