用于即时护理病理学的双轴共聚焦显微镜。
Dual-axis confocal microscopy for point-of-care pathology.
作者信息
Wei Linpeng, Yin Chengbo, Liu Jonathan T C
机构信息
Department of Mechanical Engineering, University of Washington, Seattle, WA 98195 USA, JTCL is also with the Department of Pathology at the University of Washington.
出版信息
IEEE J Sel Top Quantum Electron. 2019 Jan-Feb;25(1). doi: 10.1109/JSTQE.2018.2854572. Epub 2018 Jul 25.
Abstract
Dual-axis confocal (DAC) microscopy is an optical imaging modality that utilizes simple low-numerical aperture (NA) lenses to achieve effective optical sectioning and superior image contrast in biological tissues. The unique architecture of DAC microscopy also provides some advantages for miniaturization, facilitating the development of endoscopic and handheld DAC systems for imaging. This article reviews the principles of DAC microscopy, including its differences from conventional confocal microscopy, and surveys several variations of DAC microscopy that have been developed and investigated as non-invasive real-time alternatives to conventional biopsy and histopathology.
摘要
双轴共聚焦（DAC）显微镜是一种光学成像方式，它利用简单的低数值孔径（NA）透镜在生物组织中实现有效的光学切片和出色的图像对比度。DAC显微镜独特的架构也为小型化提供了一些优势，有助于开发用于成像的内窥镜和手持式DAC系统。本文回顾了DAC显微镜的原理，包括其与传统共聚焦显微镜的差异，并概述了已开发和研究的几种DAC显微镜变体，它们可作为传统活检和组织病理学的非侵入性实时替代方法。
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1
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Nat Biomed Eng. 2017 Jul;1(7). doi: 10.1038/s41551-017-0084. Epub 2017 Jun 26.
2
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Sci Rep. 2017 Oct 31;7(1):14404. doi: 10.1038/s41598-017-13735-z.
3
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J Biomed Opt. 2017 May 1;22(5):56008. doi: 10.1117/1.JBO.22.5.056008.
4
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J Biomed Opt. 2017 Apr 1;22(4):46005. doi: 10.1117/1.JBO.22.4.046005.
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IEEE Trans Med Imaging. 2017 Jul;36(7):1482-1490. doi: 10.1109/TMI.2017.2673022. Epub 2017 Feb 23.
6
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7
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8
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