Sung Kung-Bin, Liang Chen, Descour Michael, Collier Tom, Follen Michele, Richards-Kortum Rebecca
Department of Biomedical Engineering, University of Texas at Austin, 78712, USA.
IEEE Trans Biomed Eng. 2002 Oct;49(10):1168-72. doi: 10.1109/TBME.2002.803524.
We have built a fiber-optic confocal reflectance microscope capable of imaging human tissues in near real time. Miniaturization of the objective lens and the mechanical components for positioning and axially scanning the objective enables the device to be used in inner organs of the human body. The lateral resolution is 2 micrometers and axial resolution is 10 micrometers. Confocal images of fixed tissue biopsies and the human lip in vivo have been obtained at 15 frames/s without any fluorescent stains. Both cell morphology and tissue architecture can be appreciated from images obtained with this microscope.
我们构建了一种能够对人体组织进行近实时成像的光纤共聚焦反射显微镜。物镜以及用于物镜定位和轴向扫描的机械部件的小型化，使得该设备能够用于人体内部器官。横向分辨率为2微米，轴向分辨率为10微米。在未使用任何荧光染料的情况下，已以每秒15帧的速度获得了固定组织活检样本和人体嘴唇的共聚焦图像。通过该显微镜获得的图像能够清晰呈现细胞形态和组织结构。
