氯二氨基-s-三嗪的酶促降解
Enzymatic degradation of chlorodiamino-s-triazine.
作者信息
Seffernick Jennifer L, Shapir Nir, Schoeb Michael, Johnson Gilbert, Sadowsky Michael J, Wackett Lawrence P
机构信息
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St. Paul, Minnesota 55108, USA.
出版信息
Appl Environ Microbiol. 2002 Sep;68(9):4672-5. doi: 10.1128/AEM.68.9.4672-4675.2002.
Abstract
2-Chloro-4,6-diamino-s-triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4-amino-6-hydroxy-s-triazine (CAOT). CAOT underwent dechlorination via hydroxyatrazine ethylaminohydrolase (AtzB) to yield ammelide. This represents a newly discovered dechlorination reaction for AtzB. Ammelide was subsequently hydrolyzed by N-isopropylammelide isopropylaminohydrolase to produce cyanuric acid, a compound metabolized by a variety of soil bacteria.
摘要
2-氯-4,6-二氨基-s-三嗪（CAAT）是土壤中莠去津生物降解的一种代谢产物。莠去津氯水解酶（AtzA）催化莠去津的脱氯反应，但对CAAT无反应活性。在本研究中，与AtzA有98%同源性的三聚氰胺脱氨酶（TriA）催化CAAT的脱氨基反应，生成2-氯-4-氨基-6-羟基-s-三嗪（CAOT）。CAOT通过羟基莠去津乙氨基水解酶（AtzB）进行脱氯反应，生成氨茴内酰胺。这代表了AtzB一种新发现的脱氯反应。随后，氨茴内酰胺被N-异丙基氨茴内酰胺异丙氨基水解酶水解生成氰尿酸，氰尿酸是一种可被多种土壤细菌代谢的化合物。
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