一株侧耳菌（Pleurotus pulmonarius）对莠去津的代谢产物及其分离鉴定，2-氯-4-乙氨基-6-（1-羟丙基）氨基-1,3,5-三嗪。
Isolation and Characterization of a Novel Atrazine Metabolite Produced by the Fungus Pleurotus pulmonarius, 2-Chloro-4-Ethylamino-6-(1-Hydroxyisopropyl)Amino-1,3,5-Triazine.
机构信息
Migal Technological Center, Kiryat Shmona 10200, Institute of Soil and Water, Volcani Center, Bet Dagan, and Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel.
出版信息
Appl Environ Microbiol. 1993 Dec;59(12):4342-6. doi: 10.1128/aem.59.12.4342-4346.1993.
Abstract
The white rot fungus Pleurotus pulmonarius exhibited metabolism of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) in liquid culture, producing the dealkylated metabolites desethylatrazine, desisopropylatrazine, and desethyl-desisopropylatrazine. A fourth, unknown metabolite was also produced. It was isolated and was identified as 2-chloro-4-ethylamino-6-(1-hydroxyisopropyl)amino-1,3,5-triazine by gas chromatography-mass spectrometry, Fourier transformed infrared spectroscopy, and H nuclear magnetic resonance analysis. The structure of this metabolite was confirmed by chemical synthesis of the compound and comparison with the fungally produced metabolite.
摘要
白腐菌糙皮侧耳（Pleurotus pulmonarius）在液体培养中表现出对莠去津（2-氯-4-乙氨基-6-异丙氨基-1,3,5-三嗪）的代谢作用，生成脱烷基代谢物去乙基莠去津、去异丙基莠去津和去乙基-去异丙基莠去津。还产生了第四个未知代谢物。通过气相色谱-质谱联用、傅里叶变换红外光谱和 H 核磁共振分析将其分离并鉴定为 2-氯-4-乙氨基-6-（1-羟异丙基）氨基-1,3,5-三嗪。该代谢物的结构通过该化合物的化学合成和与真菌产生的代谢物进行比较得到确认。
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