一种新出现的人类病原体——血清型V组无乳链球菌的全基因组序列及比较基因组分析
Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae.
作者信息
Tettelin Herve, Masignani Vega, Cieslewicz Michael J, Eisen Jonathan A, Peterson Scott, Wessels Michael R, Paulsen Ian T, Nelson Karen E, Margarit Immaculada, Read Timothy D, Madoff Lawrence C, Wolf Alex M, Beanan Maureen J, Brinkac Lauren M, Daugherty Sean C, DeBoy Robert T, Durkin A Scott, Kolonay James F, Madupu Ramana, Lewis Matthew R, Radune Diana, Fedorova Nadezhda B, Scanlan David, Khouri Hoda, Mulligan Stephanie, Carty Heather A, Cline Robin T, Van Aken Susan E, Gill John, Scarselli Maria, Mora Marirosa, Iacobini Emilia T, Brettoni Cecilia, Galli Giuliano, Mariani Massimo, Vegni Filippo, Maione Domenico, Rinaudo Daniela, Rappuoli Rino, Telford John L, Kasper Dennis L, Grandi Guido, Fraser Claire M
机构信息
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
出版信息
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12391-6. doi: 10.1073/pnas.182380799. Epub 2002 Aug 28.
The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.
无乳链球菌是美国和欧洲新生儿细菌性败血症、肺炎和脑膜炎的主要病因，其2,160,267 bp的基因组序列预计编码2,175个基因。通过对无乳链球菌、肺炎链球菌、化脓性链球菌以及其他已完成全基因组测序的菌株进行基因组比较，鉴定出了链球菌属和无乳链球菌特有的基因。这些计算机分析，再结合使用全基因组微阵列对已测序的V血清型菌株2603 V/R和来自几种血清型的19株无乳链球菌进行的比较基因组杂交实验，揭示了无乳链球菌菌株间的遗传异质性，即使是同一血清型的菌株，并为毒力机制的进化提供了见解。
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