Pohl Barbara S, Knöchel Sigrun, Dillinger Karin, Knöchel Walter
Abteilung Biochemie, Universität Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany.
Mech Dev. 2002 Sep;117(1-2):283-7. doi: 10.1016/s0925-4773(02)00184-3.
We report on the temporal and spatial expression pattern of two novel genes of the Xenopus fork head/winged helix family, xFoxB2 and xFoxI1c. xFoxB2 is activated at the late blastula stage and first expressed within the dorsolateral ectoderm except for the organiser territory. During gastrulation, xFoxB2 is found in two ectodermal stripes adjacent to the dorsal midline. Expression is completely down-regulated during neurulation. However, two distinct sets of cells expressing xFoxB2 re-appear in the rhombencephalon of swimming tadpoles. xFoxI1c is initially expressed at the early neurula stage in an epidermal ring around the neural field. Subsequent expression is found to be increased, and is exclusively localised to placodal precursor cells. The placodal expression remains until stage 40, when it is restricted to a distinct region in the lateral body wall behind the gills.
我们报告了非洲爪蟾叉头/翼状螺旋家族的两个新基因xFoxB2和xFoxI1c的时空表达模式。xFoxB2在囊胚后期被激活，最初在背外侧外胚层中表达，但不包括组织者区域。在原肠胚形成期间，xFoxB2出现在与背中线相邻的两条外胚层条带中。在神经胚形成期间，表达完全下调。然而，两组表达xFoxB2的不同细胞重新出现在正在游泳的蝌蚪的后脑。xFoxI1c最初在神经胚早期在神经场周围的表皮环中表达。随后发现表达增加，并且仅定位于基板前体细胞。基板表达一直持续到第40阶段，此时它被限制在鳃后方侧体壁的一个特定区域。
