Pohl Barbara S, Knöchel Walter
Department of Biochemistry, University of Ulm, Albert Einstein-Allee 11, 89081, Ulm, Germany.
Dev Genes Evol. 2004 Apr;214(4):200-5. doi: 10.1007/s00427-004-0391-7. Epub 2004 Feb 18.
xFoxJ1 and xFoxK1, two novel members of the fork head/winged helix family of transcription factors in Xenopus, have been identified by sequence homology to their mammalian orthologues. We here report the complete amino acid sequences of xFoxJ1, its pseudo-allelic version xFoxJ1', and of xFoxK1, the latter also comprising a fork-head-associated (FHA) domain. We have further analysed the temporal and spatial expression of both genes. xFoxJ1 transcripts were detected during gastrulation in the dorsal blastopore lip and in the animal half. A predominant feature of xFoxJ1 expression until the tailbud stage is its spotty pattern within the epidermis. xFoxK1 is maternally expressed and localized within the animal half during early cleavage stages. Zygotic transcription starts during neurulation and is initially restricted to neuroectoderm. As development proceeds, xFoxK1 is expressed in ectoderm- and mesoderm-derived tissues, like the branchial arches, brain, eye, otic vesicle, pronephros, somites and abdominal muscle precursors.
爪蟾中叉头/翼状螺旋转录因子家族的两个新成员xFoxJ1和xFoxK1，已通过与它们的哺乳动物直系同源物的序列同源性鉴定出来。我们在此报告xFoxJ1、其假等位基因版本xFoxJ1'以及xFoxK1的完整氨基酸序列，后者还包含一个叉头相关（FHA）结构域。我们进一步分析了这两个基因的时空表达情况。在原肠胚形成期间，在背侧胚孔唇和动物半球中检测到xFoxJ1转录本。直到尾芽期，xFoxJ1表达的一个主要特征是其在表皮内的斑点状模式。xFoxK1在母源期表达，并在早期卵裂阶段定位于动物半球。合子转录在神经胚形成期间开始，最初局限于神经外胚层。随着发育的进行，xFoxK1在外胚层和中胚层衍生的组织中表达，如鳃弓、脑、眼、耳泡、前肾、体节和腹部肌肉前体。
