木聚糖酶、β-葡聚糖酶和纤维素酶活性的测定。
Determination of xylanase, beta-glucanase, and cellulase activity.
作者信息
König Joachim, Grasser Roland, Pikor Heather, Vogel Kurt
机构信息
Biopract GmbH, Rudower Chaussee 29, 12489 Berlin, Germany.
出版信息
Anal Bioanal Chem. 2002 Sep;374(1):80-7. doi: 10.1007/s00216-002-1379-7. Epub 2002 Jul 30.
PMID:12207246
Abstract
A simple, robust and highly reproducible method for the determination of xylanase, beta-glucanase, and cellulase in commercial feed enzyme preparations is described. The method is based on measurement of reducing moieties released by the enzymes from arabinoxylan, beta-glucan, or carboxymethylcellulose (CMC) and is independent of enzyme standards.
摘要
本文描述了一种用于测定商业饲料酶制剂中木聚糖酶、β-葡聚糖酶和纤维素酶的简单、稳健且高度可重复的方法。该方法基于测量酶从阿拉伯木聚糖、β-葡聚糖或羧甲基纤维素（CMC）释放的还原基团，且不依赖于酶标准品。
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