School of Agriculture, Ludong University, Yantai 264025, China.
College of Plant Health and Medicine, Key Lab of Integrated Crop Disease and Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2023 Jun 24;24(13):10584. doi: 10.3390/ijms241310584.
Oyster mushroom spherical virus (OMSV) is a mycovirus with a positive-sense single-stranded RNA genome that infects the edible mushroom . OMSV is horizontally transferred from an infected strain to a cured strain via mycelia. The infection results in significant inhibition of mycelial growth, malformation of fruiting bodies, and yield loss in oyster mushrooms. This study successfully transferred OMSV from to . However, transmission was not successful in other species including , , , and . The successful OMSV infection in was further verified with Western blot analysis using a newly prepared polyclonal antiserum against the OMSV coat protein. Furthermore, OMSV infection reduced the mycelial growth rate of . The OMSV-infected strain demonstrated abnormal performance including twisted mushrooms or irregular edge of the cap as well as reduced yield of fruiting bodies in , compared to the OMSV-free strain. This study is the first report on the infection and pathogenicity of OMSV to the new host . The data from this study therefore suggest that OMSV is a potential threat to .
蚝蘑球形病毒（OMSV）是一种感染可食用蘑菇的正链单链 RNA 病毒。OMSV 通过菌丝在感染株和健康株之间水平传播。感染会导致菌丝生长显著抑制、子实体畸形和产量损失。本研究成功地将 OMSV 从 转移到 。然而，在其他 物种中，包括 、 、 和 ，传输并不成功。使用针对 OMSV 外壳蛋白新制备的多克隆抗血清进行 Western blot 分析，进一步验证了 中 OMSV 的成功感染。此外，OMSV 感染降低了 的菌丝生长速度。与无 OMSV 菌株相比，感染 OMSV 的菌株在 中表现出异常，如扭曲的蘑菇或帽边缘不规则以及子实体产量降低。本研究是关于 OMSV 感染新宿主 和致病性的首次报道。因此，本研究的数据表明 OMSV 可能对 构成威胁。
