Ozkur Mehtap Kilinc, Bozkurt Meral Sasoglu, Balabanli Barbaros, Aricioglu Aysel, Ilter Nilsel, Gürer Mehmet Ali, Inalöz H Serhat
Department of Pharmacology, Medical Faculty of Gaziantep University, Sarigulluk, Gaziantep/Turkey.
Photodermatol Photoimmunol Photomed. 2002 Jun;18(3):117-20. doi: 10.1034/j.1600-0781.2002.00727.x.
BACKGROUND/PURPOSE: Free oxygen radicals are involved in inflammatory skin reactions induced by ultraviolet B (UVB). In this study, the effect of a herbal antioxidant Ginkgo biloba extract (EGb 761) was investigated in UVB irradiated mice skin.
The study was carried out on four groups of mice (n = 6 in each group). The first group was a control group (G1). The second group (G2) was only exposed to acute UVB irradiation. The third group (G3) received 100 mg/kg/day of EGb 761 orally for 5 days before UVB irradiation and the fourth group (G4) was given only a single dose of EGb 761 immediately after UVB irradiation. Eighteen hours after exposing to UVB, lipid peroxide levels, and superoxide dismutase (SOD) activities were studied and UVB damage was evaluated histopathologically according to "sun-burn cell count".
The SOD activities and Malondialdehyde (MDA) levels in G2, G3 and G4 were found to be decreased significantly when compared with G1 (P < 0.05). The SOD activities of G3 and G4 were higher when compared with G2 (P < 0.05). The number of sunburn cells (SBCs) was the highest in G2.
Our results suggest that EGb 761 may have an important effect, both as a protective and therapeutic agent, in sunburn after UVB irradiation.
背景/目的：游离氧自由基参与紫外线B（UVB）诱导的皮肤炎症反应。本研究探讨了一种草本抗氧化剂银杏叶提取物（EGb 761）对UVB照射小鼠皮肤的影响。
对四组小鼠进行研究（每组n = 6）。第一组为对照组（G1）。第二组（G2）仅接受急性UVB照射。第三组（G3）在UVB照射前5天每天口服100 mg/kg的EGb 761，第四组（G4）在UVB照射后立即仅给予单剂量的EGb 761。暴露于UVB 18小时后，研究脂质过氧化物水平和超氧化物歧化酶（SOD）活性，并根据“晒伤细胞计数”进行组织病理学评估UVB损伤。
与G1组相比，G2、G3和G4组的SOD活性和丙二醛（MDA）水平显著降低（P < 0.05）。与G2组相比，G3和G4组的SOD活性更高（P < 0.05）。G2组的晒伤细胞（SBCs）数量最高。
我们的结果表明，EGb 761作为一种保护和治疗剂，可能对UVB照射后的晒伤有重要作用。
