Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
J Physiol Pharmacol. 2011 Jun;62(3):385-92.
Solar ultraviolet radiation (UV) is a major cause of non-melanoma skin cancer in humans. Photochemoprevention with natural products represents a simple but very effective strategy in the management of cutaneous neoplasia. The study investigated the protective activity of Calluna vulgaris (Cv) and red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on UVB-induced deleterious effects in SKH-1 mice skin. Forty SKH-1 mice were randomly divided into 4 groups (n=10): control, UVB irradiated, Cv + UVB irradiated, BM+UVB irradiated. Both extracts were applied topically on the skin in a dose of 4 mg/40 μl/cm(2) before UVB exposure - single dose. The effects were evaluated in skin 24 hours after irradiation through the presence of cyclobutane pyrimidine dimers (CPDs) and sunburn cells, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 levels. The antioxidant activity of BM extract was higher than those of Cv extract as determined using stable free radical DPPH assay and ABTS test. One single dose of UVB generated formation of CPDs (p<0.0001) and sunburn cells (p<0.0002) and increased the cytokine levels in skin (p<0.0001). Twenty hours following irradiation BM extract inhibited UVB-induced sunburn cells (p<0.02) and CPDs formation (p<0.0001). Pretreatment with Cv and BM extracts resulted in significantly reduced levels of IL-6 and TNF-α compared with UVB alone (p<0.0001). Our results suggest that BM extracts might be a potential candidate in preventing the damages induced by UV in skin.
太阳紫外线辐射（UV）是人类非黑色素瘤皮肤癌的主要原因。天然产物的光化学预防代表了一种简单但非常有效的皮肤肿瘤管理策略。本研究调查了普通石楠（Cv）和红葡萄种子（Vitis vinifera L，Burgund Mare 品种）（BM）提取物在体内对 SKH-1 小鼠皮肤中 UVB 诱导的有害作用的保护活性。四十只 SKH-1 只小鼠随机分为 4 组（n=10）：对照组、UVB 照射组、Cv+UVB 照射组、BM+UVB 照射组。两种提取物均在 UVB 暴露前（单次剂量）以 4mg/40μl/cm²的剂量局部应用于皮肤。照射后 24 小时通过检测环丁烷嘧啶二聚体（CPDs）和晒伤细胞、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6 水平来评估效果。使用稳定自由基 DPPH 测定法和 ABTS 试验测定，BM 提取物的抗氧化活性高于 Cv 提取物。单次剂量的 UVB 会导致 CPDs 的形成（p<0.0001）和晒伤细胞的形成（p<0.0002），并增加皮肤中的细胞因子水平（p<0.0001）。照射后 20 小时，BM 提取物抑制了 UVB 诱导的晒伤细胞（p<0.02）和 CPDs 的形成（p<0.0001）。与单独使用 UVB 相比，Cv 和 BM 提取物预处理可显著降低 IL-6 和 TNF-α 的水平（p<0.0001）。我们的结果表明，BM 提取物可能是预防皮肤中 UV 诱导损伤的潜在候选物。
