Elias W P, Uber A P, Tomita S K, Trabulsi L R, Gomes T A T
Laboratório Especial de Microbiologia, Instituto Butantan, São Paulo, SP, Brazil.
Epidemiol Infect. 2002 Aug;129(1):49-55. doi: 10.1017/s0950268802007136.
Enteroaggregative Escherichia coli (EAEC) is defined by the ability to produce aggregative adherence (AA) to cultured cells. We analysed 128 EAEC strains, isolated from children with and without diarrhoea, regarding the presence of 11 EAEC virulence genes. Seventy strains carried and 58 lacked the EAEC probe sequence; 17 probe positive and 31 probe negative strains showed variations in the AA pattern. All EAEC probe positive strains carried at least one EAEC marker; aspU (94.3%), irp2 (91.4%), and aggR (74.3%) were the most prevalent. Conversely, among the EAEC probe negative strains, 41.4% were devoid of any marker and astA predominated (44.8%). No significant statistical difference in the prevalence of any marker between cases and controls in both EAEC probe groups or AA variants was found. We suggest that the EAEC probe positive strains may have a higher pathogenic potential or alternatively, EAEC probe negative strains may harbour virulence factors as yet undescribed.
肠集聚性大肠杆菌（EAEC）的定义是能够对培养细胞产生集聚性黏附（AA）。我们分析了128株从腹泻和未腹泻儿童中分离出的EAEC菌株，检测了11种EAEC毒力基因的存在情况。70株携带而58株缺乏EAEC探针序列；17株探针阳性和31株探针阴性菌株在AA模式上存在差异。所有EAEC探针阳性菌株至少携带一种EAEC标志物；aspU（94.3%）、irp2（91.4%）和aggR（74.3%）最为常见。相反，在EAEC探针阴性菌株中，41.4%没有任何标志物，astA占主导（44.8%）。在两个EAEC探针组或AA变体的病例和对照之间，任何标志物的流行率均未发现显著统计学差异。我们认为，EAEC探针阳性菌株可能具有更高的致病潜力，或者相反，EAEC探针阴性菌株可能含有尚未描述的毒力因子。
