Rathelot J, Julien R, Canioni P, Sarda L
Biochimie. 1975;57(10):1123-30. doi: 10.1016/s0300-9084(76)80573-1.
Three molecular forms of colipase (colipases A, B and C) with the same specific activity have been isolated from an acid extract of bovine pancreas. Purification includes ammonium sulfate precipitation, ethanol treatment, chromatography on SP-Sephadex, chromatography on DEAE-cellulose and chromatography on QAE-Sephadex. The most basic form of bovine colipase (colipase A) has a molecular weight of 11,000-12,000 daltons and contains 104 residues. Its aminoacid composition is very similar to that of the intact form of porcine colipase isolated by Borgström et al. Colipases from both species have the same N-terminal residue (valine). It is likely that bovine colipases B and C represent partially degraded forms of colipase A. Their cofactor activity, however, is the same.
已从牛胰腺的酸性提取物中分离出三种具有相同比活性的共脂肪酶分子形式（共脂肪酶A、B和C）。纯化过程包括硫酸铵沉淀、乙醇处理、SP-葡聚糖凝胶柱色谱、DEAE-纤维素柱色谱和QAE-葡聚糖凝胶柱色谱。牛共脂肪酶最碱性的形式（共脂肪酶A）分子量为11,000 - 12,000道尔顿，含有104个残基。其氨基酸组成与Borgström等人分离出的完整形式的猪共脂肪酶非常相似。两种物种的共脂肪酶具有相同的N端残基（缬氨酸）。牛共脂肪酶B和C可能代表共脂肪酶A的部分降解形式。然而，它们的辅因子活性相同。
