Sternby B, Engström A, Hellman U
Biochim Biophys Acta. 1984 Sep 11;789(2):159-63. doi: 10.1016/0167-4838(84)90200-0.
Pure colipase from dogfish (Squalus acanthius) was obtained from an extract of pancreatic gland. It has a high isoelectric point (10.2) and the molecular weight was calculated to be 9108-9383. The N-terminal sequence was shown to be Gly-Leu-Phe-Leu-Asn-Leu-Ser-Ala-Gly-Glu-Leu-Cys-Val-Gly-Ser-Phe-Gln -Cys-Lys-Ser-Ser-Cys-Cys-Gln-Arg-Glu-Thr-Gly-Leu-Ser-Leu-Ala -Arg-Cys-Ala-. This sequence shows great homology with colipases from man, horse, pig and hen. There were indications of the existence of a proform of dogfish colipase. The propeptide was found to be Ala-Pro-Glu-Arg.
来自角鲨（棘鲨）的纯辅脂酶是从胰腺提取物中获得的。它具有较高的等电点（10.2），计算得出的分子量为9108 - 9383。N端序列显示为甘氨酸-亮氨酸-苯丙氨酸-亮氨酸-天冬酰胺-亮氨酸-丝氨酸-丙氨酸-甘氨酸-谷氨酸-亮氨酸-半胱氨酸-缬氨酸-甘氨酸-丝氨酸-苯丙氨酸-谷氨酰胺-半胱氨酸-赖氨酸-丝氨酸-丝氨酸-半胱氨酸-半胱氨酸-谷氨酰胺-精氨酸-谷氨酸-苏氨酸-甘氨酸-亮氨酸-丝氨酸-亮氨酸-丙氨酸-精氨酸-半胱氨酸-丙氨酸-。该序列与人、马、猪和鸡的辅脂酶具有高度同源性。有迹象表明存在角鲨辅脂酶的前体形式。发现前肽为丙氨酸-脯氨酸-谷氨酸-精氨酸。
