Yan Xiaoqun, Kita Masakazu, Minami Masato, Yamamoto Toshiro, Kuriyama Hiroko, Ohno Tomoyuki, Iwakura Yoichiro, Imanishi Jiro
Department of Microbiology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Microbiol Immunol. 2002;46(7):475-82. doi: 10.1111/j.1348-0421.2002.tb02721.x.
Because Helicobacter pylori (H. pylori) infection is a major cause of gastroduodenal diseases in humans, the eradication of H. pylori using antibiotics is very effective for the treatment of gastroduodenal diseases. However, it has recently been reported that resistance to these antibiotics is developing. In the present study, the antibacterial effect of a Kampo (traditional Japanese medicine) herbal formulation, Hochu-ekki-to (RET; Formula repletionis animalis et supletionis medii), against H. pylori was examined in vitro and in vivo. HET inhibited the growth of antibiotic-resistant strains of H. pylori as well as antibiotic-sensitive strains at a dose of 2.5 mg/ml in vitro. When 1,000 mg/kg of HET was administered orally to C57BL/6 mice for 7 days before or after inoculation with H. pylori, H. pylori in the stomach was significantly reduced in the HET-pre-treatment group compared with the control group. Furthermore, HET in combination with antibiotics completely eradicated the bacteria in mice. The expression of interferon (IFN)-gamma was induced in the gastric mucosa of the mice pre-treated with HET. There were no significant differences between the colonization of H. pylori in the control and HET treatment groups in IFN-gamma gene-deficient mice. These results suggest that the antibacterial effect of HET may be partly due to IFN-gamma induction, and that HET may be clinically useful for treatment of H. pylori infection.
由于幽门螺杆菌（H. pylori）感染是人类胃十二指肠疾病的主要病因，使用抗生素根除幽门螺杆菌对胃十二指肠疾病的治疗非常有效。然而，最近有报道称对这些抗生素的耐药性正在出现。在本研究中，对一种汉方（传统日本医学）草药制剂补中益气汤（RET；动物补剂及中剂补充方）对幽门螺杆菌的抗菌作用进行了体外和体内研究。在体外，当剂量为2.5 mg/ml时，补中益气汤抑制了幽门螺杆菌抗生素耐药菌株以及抗生素敏感菌株的生长。在接种幽门螺杆菌之前或之后，给C57BL/6小鼠口服1000 mg/kg的补中益气汤，持续7天，与对照组相比，补中益气汤预处理组胃内的幽门螺杆菌显著减少。此外，补中益气汤与抗生素联合使用完全根除了小鼠体内的细菌。在经补中益气汤预处理的小鼠胃黏膜中诱导了干扰素（IFN）-γ的表达。在IFN-γ基因缺陷小鼠中，对照组和补中益气汤治疗组幽门螺杆菌的定植没有显著差异。这些结果表明，补中益气汤的抗菌作用可能部分归因于IFN-γ的诱导，并且补中益气汤在临床上可能对治疗幽门螺杆菌感染有用。
