Aarestrup Frank Møller, Jensen Lars Bogø
Danish Veterinary Institute, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark.
Vet Microbiol. 2002 Oct 2;89(1):83-94. doi: 10.1016/s0378-1135(02)00177-3.
From 1996 to 2001 a total of 467 Staphylococcus hyicus isolates from exudative epidermitis (EE) in pigs in Denmark were examined for susceptibility to 13 different antimicrobial agents. The presence of selected genes encoding macrolide (erm(A), erm(B) and erm(C)), penicillin (blaZ), streptogramin (vat, vga, vga(B), vat(B), vat(D) and vat(E)), streptomycin (aadE) and tetracycline resistance (tet(K), tet(L), tet(M) and tet(O)) were determined in selected isolates. The occurrence of erythromycin resistance increased from 33% in 1996 to a maximum of 62% in 1997 and decreased to 26% in 2001. Resistance to sulphametazole increased from 17% in 1996 to 30% in 1998 but has since decreased to 4% in 2001. Resistance to trimethoprim increased to 51% in 1997 and decreased to 21% in 2001. Resistance to tetracycline (21-31%) remained relatively constant during 1996-2000, but increased to 47% in 2001. Resistance to penicillin (54-75%) streptomycin (33-53%) and tetracycline (21-47%) remained relatively constant over the time investigated. All 48 penicillin resistant isolates examined contained the blaZ gene and 40 (85%) of the streptomycin resistant isolates the aadE gene. It was not possible to detect any streptogramin resistance gene in four streptogramin resistant isolates. Of the 55 erythromycin resistant isolates examined, five contained erm(A), 13 erm(B), 35 erm(C) and two both erm(A) and erm(C). The presence of erm(B) was confirmed by hybridization to plasmid profiles in all 13 PCR-positive isolates. Of 52 tetracycline resistant isolates examined, two contained tet(L), 38 tet(K) and 12 both tet(K) and tet(L).
1996年至2001年期间，对丹麦猪渗出性皮炎（EE）中分离出的467株猪葡萄球菌进行了检测，以确定其对13种不同抗菌药物的敏感性。在选定的分离株中，检测了编码大环内酯类（erm(A)、erm(B)和erm(C)）、青霉素（blaZ）、链阳菌素（vat、vga、vga(B)、vat(B)、vat(D)和vat(E)）、链霉素（aadE）和四环素抗性（tet(K)、tet(L)、tet(M)和tet(O)）的特定基因的存在情况。红霉素抗性的发生率从1996年的33%上升到1997年的最高62%，并在2001年降至26%。对磺胺甲恶唑的抗性从1996年的17%上升到1998年的30%，但此后在2001年降至4%。对甲氧苄啶的抗性在1997年上升到51%，并在2001年降至21%。对四环素的抗性（21%-31%）在1996年至2000年期间保持相对稳定，但在2001年上升到47%。在所研究的时间段内，对青霉素（54%-75%）、链霉素（33%-53%）和四环素（21%-47%）的抗性保持相对稳定。所有检测的48株青霉素抗性分离株均含有blaZ基因，40株（85%）链霉素抗性分离株含有aadE基因。在4株链阳菌素抗性分离株中未能检测到任何链阳菌素抗性基因。在所检测的55株红霉素抗性分离株中，5株含有erm(A)，13株含有erm(B)，35株含有erm(C)，2株同时含有erm(A)和erm(C)。通过与质粒图谱杂交，在所有13株PCR阳性分离株中均证实了erm(B)的存在。在所检测的52株四环素抗性分离株中，2株含有tet(L)，38株含有tet(K)，12株同时含有tet(K)和tet(L)。
