Vanlerberghe G. C., Vanlerberghe A. E., McIntosh L.
Department of Botany and Division of Life Sciences, University of Toronto at Scarborough, 1265 Military Trail, Scarborough, Ontario, Canada M1C 1A4 (G.C.V., A.E.V.).
Plant Physiol. 1997 Feb;113(2):657-661. doi: 10.1104/pp.113.2.657.
With the cytochrome pathway inhibited, AOX was able to support considerable growth of cultured tobacco (Nicotiana tabacum cv Petit Havana SR1) cells but the efficiency of carbon utilization decreased dramatically. Antisense cells with decreased AOX protein did not grow, whereas sense cells with elevated AOX protein had higher growth and respiration rates than the wild type. In antisense cells a large accumulation of pyruvate resulted in aerobic ethanolic fermentation.
随着细胞色素途径受到抑制，交替氧化酶（AOX）能够支持培养的烟草（烟草品种 Petit Havana SR1）细胞的显著生长，但碳利用效率急剧下降。AOX 蛋白含量降低的反义细胞无法生长，而 AOX 蛋白含量升高的正义细胞比野生型具有更高的生长和呼吸速率。在反义细胞中，丙酮酸的大量积累导致了有氧乙醇发酵。
