Yoneyama Yoshio, Suzuki Shunji, Sawa Rintaro, Miura Atsushi, Doi Daisuke, Otsubo Yasuo, Araki Tsutomu
Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
Am J Obstet Gynecol. 2002 Sep;187(3):676-80. doi: 10.1067/mob.2002.125764.
The purpose of this study was to investigate the correlation of plasma nitric oxide levels with the expression of P-selectin on platelets in preeclampsia.
Plasma levels of nitrite and nitrate (the stable nitric oxide metabolites) and the expression of P-selectin on platelets (a platelet activation marker) were measured in 25 normal pregnant women and 25 women with preeclampsia. The effects of the inhibition of nitric oxide synthesis on the expression of P-selectin also was measured in vitro.
Plasma nitrate levels and the expression of P-selectin averaged 30.5 +/- 2.2 micromol/L and 8.9% +/- 1.1% (SEM), respectively, in preeclampsia, which was significantly higher than in normal pregnancy (P <.05). Nitric oxide synthesis inhibition in vitro significantly increased the expression of P-selectin in normal pregnancy by 284% (P <.05), which was significantly higher than in preeclampsia (156%, P <.05).
The inhibitory effects of increased nitric oxide on the expression of P-selectin is attenuated in preeclampsia, which may contribute partly to the pathogenesis of preeclampsia.
本研究旨在探讨子痫前期患者血浆一氧化氮水平与血小板上P-选择素表达之间的相关性。
检测了25例正常孕妇和25例子痫前期患者血浆中亚硝酸盐和硝酸盐（一氧化氮的稳定代谢产物）水平以及血小板上P-选择素（一种血小板活化标志物）的表达。还在体外检测了一氧化氮合成抑制对P-选择素表达的影响。
子痫前期患者血浆硝酸盐水平和P-选择素表达平均分别为30.5±2.2微摩尔/升和8.9%±1.1%（标准误），显著高于正常妊娠（P<.05）。体外一氧化氮合成抑制使正常妊娠中P-选择素表达显著增加284%（P<.05），显著高于子痫前期（156%，P<.05）。
子痫前期中一氧化氮增加对P-选择素表达的抑制作用减弱，这可能部分促成了子痫前期的发病机制。
