Kunicka A, Lenartowska I, Sielicka M
Probl Med Wieku Rozwoj. 1975;5:155-60.
The study has aimed at investigating the blastic transformation of peripheral blood lymphocytes in patients with pituitary dwarfism before, during and after the treatment with human growth hormone. The reactivity of lymphocytes in vitro was studied in cultures stimulated with phytohemagglutinin-M of the Difco Company (PHA), in cultures stimulated with human growth hormone (HGH), and in cultures non-stimulated in vitro. The investigations were carried out in 34 patients with pituitary dwarfism at the age of 7 to 19 years. The control group was made up of 16 healthy children, 8 to 15 years old who had never been administered HGH. The patients were divided into groups according to the circumstance whether it was the first or the subsequent contact with HGH, and according to the periods at which the blood samples were collected during after therapy. It was found that the capability of lymphocytes to blastic transformation after stimulation with a specific stimulus (HGH) in patients with pituitary dwarfism in comparison with the reaction of healthy children is reduced (Fig. 2). The ability of the transformation of lymphocytes in this case shows selectivity towards the stimulus because the inhibiting of the response was noted only after the stimulation with HGH, but not after stimulation with PHA. During the treatment with HGH, allergization of the body appears; this is evidenced by the increase in the percentage of blastic cells in autotransformation (Fig. 1).
该研究旨在调查垂体性侏儒症患者在使用人生长激素治疗前、治疗期间及治疗后的外周血淋巴细胞的原始细胞转化情况。在由Difco公司的植物血凝素-M（PHA）刺激的培养物、由人生长激素（HGH）刺激的培养物以及未进行体外刺激的培养物中，研究了淋巴细胞在体外的反应性。对34名7至19岁的垂体性侏儒症患者进行了调查。对照组由16名8至15岁从未使用过HGH的健康儿童组成。根据患者是首次还是再次接触HGH的情况，以及在治疗后采集血样的时期，将患者分成不同组。结果发现，与健康儿童的反应相比，垂体性侏儒症患者在受到特定刺激（HGH）后淋巴细胞的原始细胞转化能力降低（图2）。在这种情况下，淋巴细胞的转化能力对刺激具有选择性，因为仅在受到HGH刺激后才观察到反应受到抑制，而在受到PHA刺激后则未观察到。在使用HGH治疗期间，机体出现过敏反应；这可通过自身转化中原始细胞百分比的增加得到证明（图1）。
