Ohara Masayuki, Kawashima Yuzo, Watanabe Hiromitsu, Kitajima Shunichi
Otsuka Pharmaceutical Factory, Inc., Muya-cho, Naruto, Tokushima 772-8601, Japan.
Int J Mol Med. 2002 Oct;10(4):407-11.
To explore the possibility of using blue light for extracorporeal circulation therapy in patients with leukemia, the effects of blue light on cell growth in vitro and in extracorporeally circulated blood of rats with leukemia were evaluated. When HL60 cells circulated extracorporeally using a peristaltic pump were exposed to blue light for 5 h, the growth of the cells was found to be markedly suppressed. Then, the blood of rats with erythroblastic leukemia, induced by the administration of tap water containing l-ethyl-l-nitrosourea (ENU) for 9-16 weeks, was circulated extracorporeally and exposed to blue light for 3 h. Lymphocytes were separated from the peripheral blood immediately after the end of blue-light-exposure and incubated for 7 days. The growth of leukemic cells was found to be significantly suppressed following exposure to blue light, whereas the growth of normal lymphocytes was unaffected. These findings suggest that cancer cells may be more sensitive to blue light than normal cells.
为了探索蓝光用于白血病患者体外循环治疗的可能性，评估了蓝光对白血病大鼠体外循环血液及体外细胞生长的影响。当使用蠕动泵进行体外循环的HL60细胞暴露于蓝光下5小时后，发现细胞生长受到明显抑制。然后，对通过饮用含1-乙基-1-亚硝基脲（ENU）的自来水9 - 16周诱导产生红白血病的大鼠血液进行体外循环，并暴露于蓝光下3小时。蓝光照射结束后立即从外周血中分离淋巴细胞并培养7天。发现暴露于蓝光后白血病细胞的生长受到显著抑制，而正常淋巴细胞的生长未受影响。这些发现表明癌细胞可能比正常细胞对蓝光更敏感。
