Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Cancer Center, Zhejiang University School of Medicine, Hangzhou, 310006, Zhejiang, China.
College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
Med Oncol. 2022 Feb 12;39(5):52. doi: 10.1007/s12032-022-01650-x.
Light-emitting diode (LED)-based therapies, particularly blue LEDs with wavelengths of 400-500 nm, have shown beneficial results in several cancers, including melanoma, lymphoid cells, and skin tumors. In this study, the cell viability and apoptosis of Kasumi-1 cells treated by blue light (BL) irradiation have been explored. Firstly, BL can specially inhibit the proliferation and promote the apoptosis of Kasumi-1 cells. Furthermore, the apoptosis was triggered by the production of reactive oxygen species and the decline of mitochondrial membrane potential which was regulated by the ratio of Bcl-2(Bcl-xL)/Bax; BL caused the cells' final apoptosis accompanied with the increased cleavage of caspase-3 and poly-ADP-ribose polymerase. Finally, BL induced the degradation of AML1-ETO dependent on the activation of caspase-3. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of Kasumi-1 cells.
基于发光二极管（LED）的疗法，特别是波长为 400-500nm 的蓝光 LED，已在多种癌症中显示出有益的效果，包括黑色素瘤、淋巴样细胞和皮肤肿瘤。在这项研究中，探讨了蓝光（BL）照射对 Kasumi-1 细胞的细胞活力和细胞凋亡的影响。首先，BL 可以特异性地抑制增殖并促进 Kasumi-1 细胞的凋亡。此外，凋亡是由活性氧的产生和线粒体膜电位的下降触发的，这是由 Bcl-2(Bcl-xL)/Bax 的比值调节的；BL 导致细胞最终凋亡，并伴随着 caspase-3 和多聚 ADP-核糖聚合酶的切割增加。最后，BL 诱导了 AML1-ETO 的降解，这依赖于 caspase-3 的激活。这些结果有助于建立一种低毒性、高效率的 BL 照射策略，用于临床治疗 Kasumi-1 细胞。
