Koella J C, Sørense F L
Laboratoire de Parasitologie Evolutive, UMR 7103, Université Pierre & Marie Curie, Paris, France.
Med Vet Entomol. 2002 Sep;16(3):316-20. doi: 10.1046/j.1365-2915.2002.00381.x.
Two dietary resources - blood and sugar - were assessed for effects on the melanization immune response of the mosquito Anopheles stephensi Liston (Diptera: Culicidae) towards inoculated Sephadex beads (negatively charged C-25). This melanization is conferred by genetic factors capable of making the mosquito refractory to malaria parasites. If An. stephensi females had obtained a bloodmeal one day before inoculation with a bead, the efficacy of their immune response increased with the concentration of sugar ingested. At the highest sugar concentration (6%) tested, 38% of the mosquitoes completely melanized their bead, whereas at the lowest sugar concentration (2%), none of the mosquitoes were able to melanize their bead completely. Among mosquitoes not having a bloodmeal, the immuno-competence was low (c. 9% of the mosquitoes completely melanized their bead) and independent of sugar concentration. The observed interaction between these two resources indicates that both resources are required for the Anopheles female to develop an effective melanization immune response.
评估了两种饮食来源——血液和糖分——对接种葡聚糖珠（带负电荷的C - 25）的斯氏按蚊（双翅目：蚊科）黑化免疫反应的影响。这种黑化由能够使蚊子对疟原虫产生抗性的遗传因素引起。如果斯氏按蚊雌蚊在接种珠子前一天吸食了血液，其免疫反应的效力会随着摄入糖分浓度的增加而增强。在测试的最高糖分浓度（6%）下，38%的蚊子使它们的珠子完全黑化，而在最低糖分浓度（2%）下，没有一只蚊子能够使它们的珠子完全黑化。在没有吸食血液的蚊子中，免疫能力较低（约9%的蚊子使它们的珠子完全黑化）且与糖分浓度无关。这两种饮食来源之间观察到的相互作用表明，这两种来源都是按蚊雌蚊产生有效黑化免疫反应所必需的。
