Gorman M J, Cornel A J, Collins F H, Paskewitz S M
Entomology Department, University of Wisconsin, Madison 53706, USA.
Exp Parasitol. 1996 Dec;84(3):380-6. doi: 10.1006/expr.1996.0126.
A Plasmodium-refractory strain of Anopheles gambiae that melanizes ookinetes and intrathoracically inoculated CM-Sephadex beads was mated to a Plasmodium-susceptible strain that does not melanize the parasite or the beads. The F1 progeny were then backcrossed to the susceptible strain. Backcross progeny were given a blood meal containing infective Plasmodium cynomolgi B, and the parasites were allowed to develop for 6-7 days, at which time the infected mosquitoes were injected with CM-Sephadex beads. The next day the mosquitoes were dissected and the beads were scored for degree of melanization while the parasites were scored for degree of encapsulation. A Spearman rank order correlation test of the degree of correlation between the bead melanization phenotype and the parasite encapsulation phenotype gave a correlation coefficient of 0.74 (P < 0.01). This strong correlation between the two melanization responses suggests that the mechanisms for differential bead and parasite melanization of these two mosquito strains share at least one major gene.
将对疟原虫具抗性的冈比亚按蚊品系（该品系会使动合子和经胸腔接种的CM-葡聚糖凝胶珠黑化）与对疟原虫敏感的品系（该品系不会使寄生虫或珠子黑化）进行杂交。然后将F1代子代与敏感品系回交。给回交子代喂食含有感染性食蟹猴疟原虫B的血餐，让寄生虫发育6至7天，此时给受感染的蚊子注射CM-葡聚糖凝胶珠。第二天解剖蚊子，对珠子的黑化程度进行评分，同时对寄生虫的包囊化程度进行评分。对珠子黑化表型与寄生虫包囊化表型之间的相关程度进行斯皮尔曼等级相关检验，得到的相关系数为0.74（P < 0.01）。这两种黑化反应之间的强相关性表明，这两种蚊子品系对珠子和寄生虫进行差异化黑化的机制至少共享一个主要基因。
