粪肠球菌致龋菌株中质粒脱氧核糖核酸的分析：一种鉴定隐蔽质粒上遗传决定因素的方法。
Analysis of plasmid deoxyribonucleic acid in a cariogenic strain of Streptococcus faecalis: an approach to identifying genetic determinants on cryptic plasmids.
作者信息
Oliver D R, Brown B L, Clewell D B
出版信息
J Bacteriol. 1977 May;130(2):759-65. doi: 10.1128/jb.130.2.759-765.1977.
Abstract
Streptococcus faecalis strains ND539 and OG1 have been previously shown to be cariogenic in gnotobiotic animals. Deoxyribonucleic acid analyses have revealed the presence of a single 26-megadalton plasmid designated pAM539 in the former strain, whereas the latter strain was found to be plasmid-free. By gene transfer experiments, it was possible to construct isogenic pairs of strains that differed only with regard to the presence or absence of pAM539. Comparative studies of isogenic pairs showed that the presence of pAM539 conferred bacterial sensitivity to a bacteriocin produced by S. faecalis strain 5952.
摘要
粪肠球菌菌株ND539和OG1先前已被证明在无菌动物中具有致龋性。脱氧核糖核酸分析显示，前一种菌株中存在一个名为pAM539的单一26兆道尔顿质粒，而后一种菌株则无质粒。通过基因转移实验，有可能构建仅在pAM539存在与否方面存在差异的同基因菌株对。对同基因菌株对的比较研究表明，pAM539的存在使细菌对粪肠球菌菌株5952产生的一种细菌素敏感。
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