Kurup V P, Fink J N
J Clin Microbiol. 1975 Jul;2(1):55-61. doi: 10.1128/jcm.2.1.55-61.1975.
A scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. Eighty strains, 10 Micropolyspora faeni, 6 Saccharomonospora viridis, 52 Thermoactinomyces candidus, 7 T. vulgaris, 4 T. sacchari, and 1 T. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. In addition to the cultural and microscopic morphology in various media, each strain was subjected to an array of biochemical tests. These tests included decomposition of tyrosine, xanthine, hypoxanthine, gelatin, casein, esculin, and arbutin. Using a rapid thin-layer chromatography method, the isomer of diaminopimelic acid and sugar in the whole cell hydrolysate were studied. The thermophilic actinomycetes can be identified in a reasonable period of time using a combination of all these tests.
已开发出一种用于鉴定与过敏性肺炎相关的嗜热放线菌的方案。研究了80株菌株，其中10株微小多孢菌、6株绿色糖单孢菌、52株白色嗜热放线菌、7株普通嗜热放线菌、4株甘蔗嗜热放线菌和1株双歧嗜热放线菌，这些菌株要么是从患者环境中分离出来的，要么是作为正宗菌株获得的。除了在各种培养基中的培养和微观形态外，每个菌株都进行了一系列生化试验。这些试验包括酪氨酸、黄嘌呤、次黄嘌呤、明胶、酪蛋白、七叶苷和熊果苷的分解。使用快速薄层色谱法，研究了全细胞水解物中二氨基庚二酸和糖的异构体。结合所有这些试验，可以在合理的时间内鉴定嗜热放线菌。
