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在合成培养基中生长的费氏小多孢菌的细胞外抗原。

Extracellular antigens of Micropolyspora faeni grown in synthetic medium.

作者信息

Kurup V P, Fink J N

出版信息

Infect Immun. 1977 Feb;15(2):608-13. doi: 10.1128/iai.15.2.608-613.1977.

DOI:10.1128/iai.15.2.608-613.1977
PMID:66202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC421410/
Abstract

Extracellular antigens were prepared by growing Micropolyspora faeni in a new synthetic medium (SMSL), and this antigenic preparation was compared with antigens obtained by the double-dialysis method. These two preparations were tested against pooled sera from farmer's lung patients and rabbits immunized with these antigens by the agar-gel double-diffusion, immunoelectrophoresis, and two-dimensional immunoelectrophoresis methods. The immunological cross-reactivity between M. faeni strains of diverse origin and between other species of thermophilic actinomycetes associated with hypersensitivity pneumonitis was also investigated. The usefulness of two-dimensional immunoelectrophoresis in the comparison and standardization of antigens is discussed.

摘要

通过在一种新的合成培养基（SMSL）中培养费氏小多孢菌来制备细胞外抗原，并将这种抗原制剂与通过双透析法获得的抗原进行比较。通过琼脂凝胶双向扩散、免疫电泳和二维免疫电泳方法，用这些制剂检测农民肺患者的混合血清以及用这些抗原免疫的兔子血清。还研究了不同来源的费氏小多孢菌菌株之间以及与过敏性肺炎相关的其他嗜热放线菌物种之间的免疫交叉反应性。讨论了二维免疫电泳在抗原比较和标准化方面的实用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/58cca2aeaf16/iai00206-0270-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/9e15a00f2649/iai00206-0269-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/ad8e8acf5dca/iai00206-0270-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/58cca2aeaf16/iai00206-0270-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/9e15a00f2649/iai00206-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/34611e605e48/iai00206-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/988c3576bfa6/iai00206-0269-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/d2d8e8c43462/iai00206-0269-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/4d05cea2810a/iai00206-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/bd8644bf2074/iai00206-0270-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/ad8e8acf5dca/iai00206-0270-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/365b/421410/58cca2aeaf16/iai00206-0270-d.jpg

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Extracellular antigens of Micropolyspora faeni grown in synthetic medium.在合成培养基中生长的费氏小多孢菌的细胞外抗原。
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