Sharpe G L, Larsson K S, Liedén S A
Nutr Metab. 1975;18(5-6):245-57. doi: 10.1159/000175602.
Pregnant Sprague-Dawley rats, a fed a rapeseed protein diet (containing 0.2 mg glucosinolates/g protein concentrate) from day 0, showed no teratological effects on the 18th day. However, rats which were permitted to deliver, developed anorexia and weight loss after day 18. A reddish discharge, not blood, from the nose stained the fur of most animals fed rapeseed protein. A similar discharge developed in dams fed on lab chow but fasted after day 18. At delivery, dams would neglect the newborn during the first 24 h but would then resume their diet and litter care. Surviving litters of rapeseed-fed animals were comparable to controls in weight after 3 weeks. Vitamin supplementation did not prevent these effects. Force feeding the diet by gavage aggravated these toxic effects and prolonged the gestation period. No toxic effects were seen until day 18 of gestation when the rapeseed protein diet was fed to rats 3--6 weeks before mating. Control rats given glucosinolates by gavage did not show any adverse effects. The rapeseed protein diet had no effect on NMRI mice during pregnancy and on litter care up to 3 weeks.
怀孕的斯普拉格-道利大鼠从第0天开始喂食菜籽蛋白饮食（每克蛋白质浓缩物含0.2毫克硫代葡萄糖苷），在第18天时未显示出致畸作用。然而，分娩的大鼠在第18天后出现厌食和体重减轻。大多数喂食菜籽蛋白的动物鼻子流出一种非血性的微红分泌物，弄脏了毛发。喂食实验室饲料但在第18天后禁食的母鼠也出现了类似的分泌物。分娩时，母鼠在最初24小时会忽视新生幼崽，但之后会恢复饮食并照顾幼崽。喂食菜籽的动物存活下来的幼崽在3周后体重与对照组相当。补充维生素并不能预防这些影响。通过灌胃强制喂食这种饮食会加重这些毒性作用并延长妊娠期。在交配前3至6周给大鼠喂食菜籽蛋白饮食，直到妊娠第18天才出现毒性作用。通过灌胃给对照大鼠喂食硫代葡萄糖苷未显示出任何不良反应。菜籽蛋白饮食对怀孕期的NMRI小鼠以及长达3周的幼崽护理没有影响。
