Endho N, Hashimoto Y
Immunology. 1981 Nov;44(3):499-507.
Mixed culture of nylon non-adherent (T-enriched) spleen cells or lymph node cells of ACI/N or F344 rats with mitomycin C-treated autologous spleen cells resulted in the increased DNA synthesis of the responder T cells when they were cultured in medium containing foetal calf serum (FCS) or bovine serum albumin. This autologous mixed lymphocyte reaction (AMLR), however, proceeds little when the cells were cultured in medium containing autologous or allogeneic rat serum, irrespective of the responder:stimulator cell ratio and to the culture period. Furthermore, the AMLR response in FCS was greatly reduced if a relatively large number of macrophages were present in the responder cell population. These results suggest that the AMLR response is unlikely to occur in a physiological condition or in vivo.
将 ACI/N 或 F344 大鼠的尼龙非黏附（富含 T 细胞）脾细胞或淋巴结细胞与丝裂霉素 C 处理的自体脾细胞进行混合培养，当在含有胎牛血清（FCS）或牛血清白蛋白的培养基中培养时，反应性 T 细胞的 DNA 合成增加。然而，当细胞在含有自体或同种异体大鼠血清的培养基中培养时，无论反应细胞与刺激细胞的比例以及培养时间如何，这种自体混合淋巴细胞反应（AMLR）几乎不发生。此外，如果反应细胞群体中存在相对大量的巨噬细胞，FCS 中的 AMLR 反应会大大降低。这些结果表明，AMLR 反应不太可能在生理条件下或体内发生。
