Mauer S M, Steffes M W, Sutherland D E, Michael A F, Brown D M
Diabetes. 1975 Mar;24(3):280-5. doi: 10.2337/diab.24.3.280.
Diabetes was induced in Lewis rats with streptozotocin. Six to eight months later glomeruli showed mesangial thickening: IgG, IgM and C3 were seen in large quantities in the mesangium by immunofluorescent microscopy. Ten animals then had successful pancreatic transplantation resulting in normal glucose and insulin levels within one to three weeks. Biopsies obtained within the first two weeks following transplantation demonstrated a significant reduction in mesangial thickening and in mesangial staining for IgG, IgM and C3. Three to four weeks after transplantation C3 staining was no longer detected. A gradual reduction in mesangial IgG and IgM localization continued so that by nine weeks following islet transplantation only minimal staining for immunoglobulins was present. Although mesangial thickening was reduced, this abnormality could still be detected in most animals six to nine weeks after transplantation. Three rats showed improvement in glomerular morphology within two weeks despite persistent hyperglycemia. These rats had normal insulin levels at this time. Islet transplantation in inbred diabetic rats effectively returns glucose and insulin levels to normal and results in rapid regression of the light microscopic and immunopathologic glomerular lesions. These studies support the concept of reversible mesangial dysfunction in diabetic rats.
用链脲佐菌素诱导Lewis大鼠患糖尿病。6至8个月后，肾小球出现系膜增厚：通过免疫荧光显微镜检查，在系膜中可见大量IgG、IgM和C3。然后，10只动物成功进行了胰腺移植，在1至3周内血糖和胰岛素水平恢复正常。移植后前两周内获取的活检显示，系膜增厚以及系膜中IgG、IgM和C3染色显著减少。移植后3至4周，不再检测到C3染色。系膜IgG和IgM定位逐渐减少，以至于胰岛移植后9周时，仅存在极少量的免疫球蛋白染色。尽管系膜增厚有所减轻，但在移植后6至9周，大多数动物仍可检测到这种异常。3只大鼠尽管持续高血糖，但在两周内肾小球形态有所改善。此时这些大鼠的胰岛素水平正常。近交系糖尿病大鼠的胰岛移植可有效使血糖和胰岛素水平恢复正常，并导致肾小球光镜和免疫病理损伤迅速消退。这些研究支持糖尿病大鼠中可逆性系膜功能障碍的概念。
