褪黑素对猪睫状动脉血管反应的影响。

Effect of melatonin on vascular responses of porcine ciliary arteries.

作者信息

Pache Mona, Kräuchi Kurt, Haefliger Ivan O, Wirz-Justice Anna, Flammer Josef, Meyer Peter

机构信息

Basel University Eye Clinic, Basel, Switzerland.

出版信息

Curr Eye Res. 2002 Apr;24(4):313-7. doi: 10.1076/ceyr.24.4.313.8416.

DOI:10.1076/ceyr.24.4.313.8416

PMID:12324871

Abstract

PURPOSE

To investigate the effect of melatonin on isolated porcine ciliary arteries.

METHODS

Isolated porcine ciliary arteries were suspended in myograph chambers filled with modified Krebs-Ringer solution (37 degrees C; 95% O2/5% CO2) for isometric tension recording.

RESULTS

In quiescent porcine ciliary arteries with endothelium, melatonin (10(-11)--10(-5) M) evoked no change in vascular tone. The highest concentration of melatonin (10(-4 ) M) evoked a small but significant contraction. In vessels precontracted with U-46619 (10(-7) M), increasing concentrations of melatonin (10(-11)--10(-5) M) did not evoke a response. In precontracted arteries with endothelium, contractile response of vascular smooth muscle to increasing concentrations of serotonin (10(-10)--10(-5) M) and noradrenaline (10(-10)--10(-5) M) was reduced after preincubation with melatonin (10(-4) M). Melatonin (10(-4) M) did not alter the response to endothelin-1 (10(-10)--10( -7) M) and U-46619 (10(-10)--10(-6 ) M) in precontracted arteries with endothelium.

CONCLUSION

These findings demonstrate that melatonin attenuates the effect of serotonin and noradrenaline and is itself a mild vasoconstrictor in porcine ciliary arteries. The role of melatonin in human ocular circulation remains to be established.

摘要

目的

研究褪黑素对离体猪睫状动脉的作用。

方法

将离体猪睫状动脉悬挂于充满改良克雷布斯-林格溶液（37℃；95% O₂/5% CO₂）的肌动描记室中，进行等长张力记录。

结果

在有内皮的静息猪睫状动脉中，褪黑素（10⁻¹¹～10⁻⁵ M）未引起血管张力变化。最高浓度的褪黑素（10⁻⁴ M）引起轻微但显著的收缩。在用U-46619（10⁻⁷ M）预收缩的血管中，褪黑素浓度增加（10⁻¹¹～10⁻⁵ M）未引起反应。在有内皮的预收缩动脉中，用褪黑素（10⁻⁴ M）预孵育后，血管平滑肌对5-羟色胺（10⁻¹⁰～10⁻⁵ M）和去甲肾上腺素（10⁻¹⁰～10⁻⁵ M）浓度增加的收缩反应降低。褪黑素（10⁻⁴ M）未改变有内皮的预收缩动脉对内皮素-1（10⁻¹⁰～10⁻⁷ M）和U-46619（10⁻¹⁰～10⁻⁶ M）的反应。