Gruhn K, Hennig A, Richter G, Raue B
Arch Tierernahr. 1975 Mar;25?*2:119-26. doi: 10.1080/17450397509423177.
Four laying hens with anus praeternaturalis were fed 15N casein for a period of 6 experimental days. Each experimental bird thus received a 15N excess (15N') of 154.44 mg. 11.54 mg of 15N' were found in lysine 3.99 mg in histidine and 9.58 mg in arginine. 2 hens were slaughtered 48 hours after the last 15N dose; another 2 were sacrificed after 144 hrs. In this investigation the N content of the intestinal contents and in the proteins of the small and large intestine were determined. The average N content of the small intestine per hen 2.65% that of the large intestine, 2.60% and, correspondingly, the TCE soluble proportion of N was 21.8% and 20.4%. The percentage of amino acids in the contents of the small intestine was found to change relative to that of the dietary proteins, approximating the protein composition of the intestine. In the first two hens the atom % 15N' was found in the different sections of the intestine and in the intestinal contents varied between 0.14 and 0.28. The data obtained from the two hens slaughtered 144 hours after the last 15N' dose were nearly the same ranging from 0.08 to 0.10 atom % 15n'.
四只患有肛门异常的产蛋母鸡被喂食含15N的酪蛋白，为期6个实验日。每只实验鸡因此摄入了154.44毫克的15N过量（15N'）。在赖氨酸中发现11.54毫克的15N'，组氨酸中3.99毫克，精氨酸中9.58毫克。在最后一次15N剂量后48小时宰杀2只母鸡；另外2只在144小时后宰杀。在这项研究中，测定了肠内容物以及小肠和大肠蛋白质中的氮含量。每只母鸡小肠的平均氮含量为2.65%，大肠为2.60%，相应地，氮的三氯乙酸可溶性比例分别为21.8%和20.4%。发现小肠内容物中氨基酸的百分比相对于膳食蛋白质有所变化，接近肠道的蛋白质组成。在前两只母鸡中，在肠道不同部位和肠内容物中发现的15N'原子百分比在0.14至0.28之间变化。从最后一次15N'剂量后144小时宰杀的两只母鸡获得的数据几乎相同，范围为0.08至0.10原子% 15n'。
