Simmering Rainer, Taras David, Schwiertz Andreas, Le Blay Gwenaelle, Gruhl Bärbel, Lawson Paul A, Collins Matrhew D, Blaut Michael
Department of Gastrointestinal Microbiology, German Institute of Human Nutrition, Bergholz-Rehbrücke.
Syst Appl Microbiol. 2002 Aug;25(2):189-93. doi: 10.1078/0723-2020-00112.
A strain of an unidentified strictly anoxic, gram-postive, non-motile Ruminococcus-like bacterium was isolated from a human faecal sample. The organism used carbohydrates as fermentable substrates, produced acetate, succinate, and hydrogen as the major products of glucose metabolism, and possessed a G + C content of 43.3 mol%. The morphological and biochemical characteristics of the organism were consistent with its assignment to the genus Ruminococcus but it did not correspond to any recognized species of this genus. Comparative 16S rRNA gene sequencing showed the unidentified bacterium represents a previously unrecognised sub-line within the Clostridium coccoides rRNA group of organisms. The nearest relative of the unknown bacterium corresponded to Ruminococcus obeum but a 16S rRNA sequence divergence value of >3% demonstrated it represents a different species. Based on the presented findings a new species, Ruminococcus luti, is described. The type strain of Ruminococcus luti is BInIX(T) (DSM 14534T, CCUG 45635T).
从一份人类粪便样本中分离出了一株未鉴定的严格厌氧、革兰氏阳性、无运动性的类瘤胃球菌属细菌。该微生物利用碳水化合物作为可发酵底物，产生乙酸盐、琥珀酸盐和氢气作为葡萄糖代谢的主要产物，其G + C含量为43.3摩尔%。该微生物的形态和生化特征与其归入瘤胃球菌属相符，但它并不对应于该属中任何已被认可的物种。比较16S rRNA基因测序表明，该未鉴定细菌代表了梭状芽孢杆菌rRNA菌群中一个以前未被认识的亚系。该未知细菌的最相近亲缘种对应于瘤胃球菌，但16S rRNA序列差异值>3%表明它代表一个不同的物种。基于所呈现的研究结果，描述了一个新物种——泥栖瘤胃球菌。泥栖瘤胃球菌的模式菌株是BInIX(T)（DSM 14534T，CCUG 45635T）。
