通过基于线粒体基因的碱基切除序列扫描胸腺嘧啶碱基读取器分析对人体带绦虫进行DNA鉴别诊断。
DNA differential diagnosis of human taeniid cestodes by base excision sequence scanning thymine-base reader analysis with mitochondrial genes.
作者信息
Yamasaki Hiroshi, Nakao Minoru, Sako Yasuhito, Nakaya Kazuhiro, Sato Marcello Otake, Mamuti Wulamu, Okamoto Munehiro, Ito Akira
机构信息
Department of Parasitology. Animal Laboratory for Medical Research, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa, Japan.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3818-21. doi: 10.1128/JCM.40.10.3818-3821.2002.
Abstract
For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.
摘要
对于人体带绦虫的DNA鉴别诊断，采用了以细胞色素c氧化酶亚基I和细胞色素b基因为靶点的碱基切除序列扫描胸腺嘧啶碱基法。特征性的胸腺嘧啶碱基峰图谱提供了四种不同类型，分别是牛带绦虫、亚洲带绦虫以及两种猪带绦虫基因型所特有的。如果有核苷酸序列数据库，这种方法为无需DNA测序的人体带绦虫鉴别和诊断提供了一个有用的工具。
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