Harant I, Marion-Latard F, Crampes F, de Glisezinski I, Berlan M, Stich V, Rivière D
Institut National de la Santé et de la Recherche Médicale (INSERM, Unité 317), Université Paul Sabatier, Toulouse, France.
Int J Obes Relat Metab Disord. 2002 Oct;26(10):1373-8. doi: 10.1038/sj.ijo.0802072.
The aim of the study was to investigate whether a long-lasting bout of exercise modifies the lipolytic beta- and antilipolytic-alpha(2)-adrenergic effect and the antilipolytic effect of insulin in obese subjects.
Biopsies of abdominal subcutaneous adipose tissue were performed before and immediately after 2 h exercise (at 50% of VO(2max)) on an ergometric bicycle.
Nine healthy obese male subjects (mean age 38.0+/-3.5 y; mean body mass index (BMI) 35.6+/-3.9 kg/m(2)) were included in the experiment.
:The lipolytic responsiveness to adrenaline, isoprenaline (beta-adrenergic agonist), UK-14304 (alpha(2)-adrenergic agonist) and insulin was studied in the isolated fat cell obtained by biopsies of subcutaneous adipose tissue from the peri-umbilical region before and after exercise.
After exercise, an increase was observed in spontaneous lipolytic rate, and in the lipolytic effect of isoprenaline, but no modification in the lipolytic action of adrenaline. Antilipolytic effects of UK-14304 and insulin were not changed by the single bout of exercise.
A single bout of long-term exercise increased the responsiveness of adipose tissue to beta-adrenergic stimulation of lipolysis in obese subjects.
本研究旨在调查长时间的运动是否会改变肥胖受试者脂肪分解的β-肾上腺素能效应和抗脂肪分解的α₂-肾上腺素能效应以及胰岛素的抗脂肪分解作用。
在测力计自行车上进行2小时运动（强度为最大摄氧量的50%）之前和之后立即采集腹部皮下脂肪组织活检样本。
九名健康肥胖男性受试者（平均年龄38.0±3.5岁；平均体重指数（BMI）35.6±3.9kg/m²）纳入实验。
研究了运动前后从脐周区域皮下脂肪组织活检获取的分离脂肪细胞对肾上腺素、异丙肾上腺素（β-肾上腺素能激动剂）、UK-14304（α₂-肾上腺素能激动剂）和胰岛素的脂肪分解反应性。
运动后，观察到自发脂肪分解率增加，异丙肾上腺素的脂肪分解作用增强，但肾上腺素的脂肪分解作用没有改变。单次运动并未改变UK-14304和胰岛素的抗脂肪分解作用。
单次长时间运动增加了肥胖受试者脂肪组织对β-肾上腺素能刺激脂肪分解的反应性。
