Morimoto Ken, Hasuma Tadayoshi, Koh Masae, Ohtani Shuzo, Kinoshita Hiroaki
Second Dept. of Surgery, Osaka City University Medical School.
Gan To Kagaku Ryoho. 2002 Sep;29(9):1597-605.
Six hundred and eighty-eight breast specimens including 442 breast cancers were studied to investigate the consistency and correlations between dextran charcoal assay (DCC) and enzyme immunoassay (EIA) for estrogen receptor (ER) and progesterone receptor (PgR). In DCC, ER was quantitated with competition of 16 alpha-125I-estradiol and diethyl stilbesterol, and PgR with 3H-R5020 and R5020. In EIA, kit from Abbott was used in ER and PgR quantitation. The mean age of the patients was 52 years and the mean age of patients with benign lesions was 39 years. The consistency rate was 95% for ER, and 83% for PgR, while the consistency coefficient kappa was 0.90 and 0.66, respectively. For the specimens in which the number of binding sites was calculated in both DCC (x) and EIA (y), the correlation coefficient was 0.787 and the linear regression formula was y = 0.5x + 58. For PgR, the correlation coefficient kappa was 0.612 and the regression formula was y = 2.6x + 91. In multiple regression analysis for consistency of DCC and EIA, for ER, there was an inconsistent trend for positive PgR and a consistent trend for patients in their fifties. For PgR, the trend was inconsistent for benign lesions and positive ER. In comparison with the efficacy of endocrine treatment, no responder was found in ER negative patients for both DCC and EIA. In PgR-negative patients a responder was found for both DCC and EIA. By Western blot analysis, anti-ER antibody provided in the ER.EIA kit showed affinity only for ER alpha and not for beta. In conclusion, in terms of the treatment efficacy for both ER and PgR, the current use of ER with EIA instead of DCC seems to give equivalent result, and old DCC data can be converted into the regression formula. However, PgR calculated by EIA was not equivalent with that of DCC.
对688份乳腺标本（包括442例乳腺癌标本）进行了研究，以调查葡聚糖炭吸附法（DCC）和酶免疫分析法（EIA）检测雌激素受体（ER）和孕激素受体（PgR）之间的一致性及相关性。在DCC法中，用16α-125I-雌二醇和己烯雌酚竞争定量ER，用3H-R5020和R5020竞争定量PgR。在EIA法中，使用雅培公司的试剂盒定量ER和PgR。患者的平均年龄为52岁，良性病变患者的平均年龄为39岁。ER的一致性率为95%，PgR为83%，而一致性系数kappa分别为0.90和0.66。对于在DCC法（x）和EIA法（y）中均计算了结合位点数的标本，相关系数为0.787，线性回归公式为y = 0.5x + 58。对于PgR，相关系数kappa为0.612，回归公式为y = 2.6x + 91。在DCC法和EIA法一致性的多元回归分析中，对于ER，PgR阳性存在不一致趋势，五十多岁的患者存在一致趋势。对于PgR，良性病变和ER阳性的趋势不一致。与内分泌治疗效果相比，ER阴性患者在DCC法和EIA法中均未发现反应者。在PgR阴性患者中，DCC法和EIA法均发现了反应者。通过蛋白质印迹分析，ER.EIA试剂盒中提供的抗ER抗体仅对ERα有亲和力，对β无亲和力。总之，就ER和PgR的治疗效果而言，目前使用EIA法检测ER而非DCC法似乎能得到等效结果，旧的DCC数据可转换为回归公式。然而，EIA法计算的PgR与DCC法不等效。
