Wu C W, Chang H M, Kao H L, Lui W Y, P'eng F K, Chi C W
Department of Surgery, Veterans General Hospital, Taipei, Taiwan.
Gastroenterology. 1992 May;102(5):1639-46. doi: 10.1016/0016-5085(92)91724-i.
Contents of the progesterone receptors (PgR) and estrogen receptors (ER) in 18 gastric adenocarcinoma tissues were determined using both the dextran-coated charcoal (DCC) assay and enzyme immunoassay (EIA). PgR were found in 15 cancer tissues (range, 1.0-58.8 fmol/mg protein) and 12 normal mucosal tissues (range, 1.4-26.8 fmol/mg protein) by DCC assay, whereas only 6 cancer tissues (ranged, 0.2-3.3 fmol/mg protein) and 7 normal mucosal tissues (range, 0.1-0.8 fmol/mg protein) had measurable PgR by EIA analysis. Similar results were observed for ER. DCC assay found ER in 12 cancer tissues (range, 2.9-112.6 fmol/mg protein) and 12 normal mucosal tissues (range, 1.2-36.6 fmol/mg protein), whereas EIA measured ER in 16 cancer tissues (range, 0.1-3.5 fmol/mg protein) and 15 normal mucosal tissues (range, 0.1-4.8 fmol/mg protein). No significant correlation between DCC and EIA was observed for either PgR or ER. DCC assay and its modified procedures including 5% DCC stripping of cytosol and/or the addition of sodium molybdate in buffer were simultaneously measured in 5 gastric adenocarcinoma tissues and 1 gastritis cystica polyposa tissue (a precancerous lesion). Higher receptor levels were found by the modified procedures than by conventional method. Using the DCC procedure with addition of sodium molybdate in buffer for receptor analysis, PgR and ER were found in gastric tissues in six patients, with significantly increased levels of measurable PgR. The results suggest that PgR and ER may be involved in the physiology of normal and gastric cancer tissues; their clinical implications are worthy of further study.
采用葡聚糖包被活性炭（DCC）分析法和酶免疫分析法（EIA）测定了18例胃腺癌组织中孕激素受体（PgR）和雌激素受体（ER）的含量。通过DCC分析法，在15例癌组织（范围为1.0 - 58.8 fmol/mg蛋白质）和12例正常黏膜组织（范围为1.4 - 26.8 fmol/mg蛋白质）中检测到PgR，而通过EIA分析，仅在6例癌组织（范围为0.2 - 3.3 fmol/mg蛋白质）和7例正常黏膜组织（范围为0.1 - 0.8 fmol/mg蛋白质）中检测到可测量的PgR。ER的检测结果相似。DCC分析法在12例癌组织（范围为2.9 - 112.6 fmol/mg蛋白质）和12例正常黏膜组织（范围为1.2 - 36.6 fmol/mg蛋白质）中检测到ER，而EIA在16例癌组织（范围为0.1 - 3.5 fmol/mg蛋白质）和15例正常黏膜组织（范围为0.1 - 4.8 fmol/mg蛋白质）中检测到ER。对于PgR或ER，未观察到DCC和EIA之间存在显著相关性。在5例胃腺癌组织和1例胃息肉样胃炎组织（一种癌前病变）中同时采用DCC分析法及其改良方法，包括用5% DCC对胞质溶胶进行洗脱和/或在缓冲液中添加钼酸钠。改良方法检测到的受体水平高于传统方法。采用在缓冲液中添加钼酸钠的DCC方法进行受体分析，在6例患者的胃组织中检测到PgR和ER，可测量的PgR水平显著升高。结果表明，PgR和ER可能参与正常和胃癌组织的生理过程；它们的临床意义值得进一步研究。
