Oh Hyuncheol, Mun Yeun-Ja, Im Sook-Jung, Lee Seung Yon, Song Ho Joon, Lee Ho-Sub, Woo Won-Hong
Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Korea.
Planta Med. 2002 Sep;68(9):832-3. doi: 10.1055/s-2002-34418.
Cucurbitacins 1 and 2 were isolated from the root of Trichosanthes kirilowii by tyrosinase inhibitory activity-guided fractionation. Spectroscopic analysis revealed that compounds 1 and 2 were cucurbitacin D and 23,24-dihydro-cucurbitacin D, respectively. Compounds 1 and 2 effectively inhibited the activity of tyrosinase (IC(50) = 0.18 microM and 6.7 microM, respectively), and the synthesis of melanin (IC(50) = 0.16 microM and 7.5 microM, respectively) in B16/F10 melanoma cells.
通过酪氨酸酶抑制活性导向分离法，从栝楼根中分离出葫芦素1和2。光谱分析表明，化合物1和2分别为葫芦素D和23,24-二氢-葫芦素D。化合物1和2能有效抑制B16/F10黑色素瘤细胞中酪氨酸酶的活性（IC(50)分别为0.18 microM和6.7 microM）以及黑色素的合成（IC(50)分别为0.16 microM和7.5 microM）。
