Okinawa Industrial Technology Center, 12-2 Suzaki, Uruma, Okinawa 901-2213, Japan.
J Agric Food Chem. 2011 Feb 23;59(4):1109-14. doi: 10.1021/jf1035542. Epub 2011 Jan 25.
Tyrosinase, trypsin, and tryptase are known to play important roles in melanin production of human skin. This paper describes the study of the inhibitory effect of Balanophora fungosa on melanin. The 50% EtOH extract obtained from B. fungosa indicated an inhibitory effect on mushroom tyrosinase activity with an IC(50) value of 15 μg/mL. Bioassay-guided fractionation of the active extract resulted in the isolation of four known compounds. Their structures were identified as 1-O-(E)-caffeoyl-3-O-galloyl-4,6-(S)-HHDP-β-d-glucopyranose (1), 1-O-(E)-caffeoyl-3,4,6-tri-O-galloyl-β-d-glucopyranose (2), caffeoyl-β-d-glucopyranose (3), and abietin (4) on the basis of spectroscopic analyses and comparison of their spectral data with those in the literature. Compounds 1 and 2 prevented pigmentation of melanin in a three-dimensional cultured human skin model. Furthermore, compounds 1 and 2 indicated inhibitory activities against trypsin and tryptase.
酪氨酸酶、胰蛋白酶和胰凝乳蛋白酶已知在人体皮肤黑色素的生成中发挥着重要作用。本文描述了研究石仙桃对黑色素的抑制作用。从石仙桃中获得的 50%乙醇提取物对蘑菇酪氨酸酶活性表现出抑制作用，IC50 值为 15μg/mL。对活性提取物进行基于生物活性的分步分离，得到了四个已知化合物。根据光谱分析和与文献中光谱数据的比较，它们的结构被鉴定为 1-O-(E)-咖啡酰-3-O-没食子酰-4,6-(S)-HHDP-β-d-吡喃葡萄糖（1）、1-O-(E)-咖啡酰-3,4,6-三-O-没食子酰-β-d-吡喃葡萄糖（2）、咖啡酰-β-d-吡喃葡萄糖（3）和扁柏素（4）。化合物 1 和 2 可防止三维培养的人体皮肤模型中的黑色素着色。此外，化合物 1 和 2 对胰蛋白酶和胰凝乳蛋白酶表现出抑制活性。
