Stanley P, Caillibot V, Siminovitch L
Somatic Cell Genet. 1975 Jan;1(1):3-26. doi: 10.1007/BF01538729.
Chinese hamster ovary (CHO) cells selected for resistance to the cytotoxicity of phytohemagglutin (PHA) have been found to exhibit stable alterations at their plasma membranes. The PHA-resistant (PhaR) cells bind markedly less 125I-PHA than do sensitive CHO cells and also exhibit an increased sensitivity to the cytotoxicity of concanavalin A, a lectin of different receptor specificity. Mutagenesis with ethylmethanesulfonate increases the proportion of PhaR cells 20- to 100-fold. PHA-resistant cells maintained for up to 8 months in continuous culture in the absence of the selective agent have retained the PhaR phenotype. These and other characteristics of the experimental system suggest that CHO cells selected for PHA resistance are authentic somatic cell mutants. The Pha marker appears to behave recessively in hybrids formed between PhaR and PhaS cells.
已发现，经选择对植物血凝素（PHA）细胞毒性具有抗性的中国仓鼠卵巢（CHO）细胞，其质膜表现出稳定的变化。与敏感的CHO细胞相比，PHA抗性（PhaR）细胞结合的¹²⁵I-PHA明显更少，并且对伴刀豆球蛋白A（一种具有不同受体特异性的凝集素）的细胞毒性也表现出更高的敏感性。用甲磺酸乙酯诱变可使PhaR细胞的比例增加20至100倍。在没有选择剂的情况下连续培养长达8个月的PHA抗性细胞仍保留PhaR表型。该实验系统的这些及其他特征表明，经选择获得PHA抗性的CHO细胞是真正的体细胞突变体。Pha标记在PhaR细胞和PhaS细胞形成的杂种中似乎表现为隐性。
