中国仓鼠卵巢细胞的植物血凝素抗性突变体中细胞表面糖蛋白的改变。
Altered cell surface glycoproteins in phytohemagglutinin-resistant mutants of Chinese hamster ovary cells.
作者信息
Juliano R L, Stanley P
出版信息
Biochim Biophys Acta. 1975 May 6;389(2):401-6. doi: 10.1016/0005-2736(75)90332-6.
PMID:166678
Abstract
Purified membranes from surface-labelled phytohemagglutinin-resistant (Pha(R) and wild-type chinese hamster ovary cells have been analysed by sodium dodecyl sulphate gel electrophoresis. Gel patterns were compared for cells labelled via galactose oxidase and B-3H4 or lactoperoxidase and radioactive iodide. The results suggest that Pha-R cells are altered in the carbohydrate portion of a number of their membrane glycoproteins.
摘要
已通过十二烷基硫酸钠凝胶电泳分析了来自表面标记的抗植物血凝素（Pha(R)）和野生型中国仓鼠卵巢细胞的纯化膜。比较了通过半乳糖氧化酶和B-3H4或过氧化物酶和放射性碘标记的细胞的凝胶图谱。结果表明，Pha-R细胞的许多膜糖蛋白的碳水化合物部分发生了改变。
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