Wright J A
J Cell Biol. 1973 Mar;56(3):666-75. doi: 10.1083/jcb.56.3.666.
Lines of Chinese hamster ovary cells resistant to the lectins concanavalin A (Con A) and phytohemagglutinin-P (PHA-P) have been isolated and characterized. Lines were isolated by a stepwise, a single-step, or a cycling single-step procedure, from both mutagen-treated and untreated cultures. The resistant lines showed a higher efficiency of colony formation in the presence of the appropriate lectin than did the wild-type parental line. The cell lines resistant to Con A did not exhibit any detectable cross resistance to PHA-P, nor did the PHA-resistant cells exhibit cross resistance to Con A. The toxicity of Con A from the wild-type and Con A-resistant lines was reduced in the presence of methyl alpha-D-glucopyranoside; this effect was not seen with the PHA-resistant line. Using (125)I-labeled Con A, it was found that Con A was bound preferentially to the surface of intact cells, and that the amount of labeled Con A bound to intact cells was similar for the wild-type and lectin-resistant lines. The Con A-resistant lines were found to be more susceptible to the toxic effects of a number of different compounds, including cyclic AMP and its dibutyryl derivative, sodium butyrate, high concentrations of glucose, phenethyl alcohol, phenol, ouabain, and testosterone. It appears that, in these lines, acquisition of resistance to Con A gave rise to pleiotropic effects which were detected by changes in the sensitivity of the cells to a variety of agents.
已分离并鉴定出对凝集素刀豆球蛋白A（Con A）和植物血凝素-P（PHA-P）具有抗性的中国仓鼠卵巢细胞系。通过逐步、单步或循环单步程序，从经诱变处理和未经处理的培养物中分离出细胞系。在存在适当凝集素的情况下，抗性细胞系比野生型亲代细胞系表现出更高的集落形成效率。对Con A具有抗性的细胞系对PHA-P未表现出任何可检测到的交叉抗性，对PHA具有抗性的细胞系对Con A也未表现出交叉抗性。在α-D-甲基吡喃葡萄糖苷存在的情况下，野生型和Con A抗性细胞系的Con A毒性降低；PHA抗性细胞系未观察到这种效应。使用（125）I标记的Con A，发现Con A优先结合完整细胞的表面，并且野生型和凝集素抗性细胞系结合到完整细胞上的标记Con A量相似。发现Con A抗性细胞系对多种不同化合物的毒性作用更敏感，包括环磷酸腺苷及其二丁酰衍生物、丁酸钠、高浓度葡萄糖、苯乙醇、苯酚、哇巴因和睾酮。在这些细胞系中，对Con A抗性的获得似乎产生了多效性效应，这些效应通过细胞对多种试剂敏感性的变化得以检测。
